GKE-5008
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Endoglycosidases

Part Number:
GKE-5008

AdvanceBio Endo F2, recombinant (formerly ProZyme). Enzyme cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Includes 5X reaction buffer, which when diluted gives 200 mM sodium acetate pH 4.5.

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Specifications
Enzyme Applications
  • Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins.
Enzyme Formulation
  • 10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase.
Enzyme Specificity
  • The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide, or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.
Enzyme Unit Definition
  • One milliunit is defined as the amount of enzyme that will hydrolyze 1 nM of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C.
Unit
  • 6 mU
Volume
  • 60 µL
pH Optimum
  • 4.5
Support
Safety Data Sheets

AdvanceBio Endo F2, recombinant

AdvanceBio Endo F2, recombinant, 6 mU, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • Portuguese
  • 15-Jul-2019
  • 289.97 Kb
  • PDF

AdvanceBio Endo F2, recombinant

AdvanceBio Endo F2, recombinant, 6 mU, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 292.39 Kb
  • PDF

AdvanceBio Endo F2, recombinant

AdvanceBio Endo F2, recombinant, 6 mU, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 285.70 Kb
  • PDF

AdvanceBio Endo F2, recombinant

AdvanceBio Endo F2, recombinant, 6 mU, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 285.83 Kb
  • PDF

AdvanceBio Endo F2, recombinant

AdvanceBio Endo F2, recombinant, 6 mU, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 339.62 Kb
  • PDF

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